Bioreactor ppt


A Bioreactor is defined as a closed system used for bioprocessing (flask, roller bottle, tank, vessel, or other container), which supports the growth of cells, mammalian or bacterial, in a culture medium. A bacterial reaction usually is said to take place in a fermenter, and cell culture in a bioreactor.
  • Bioreactor – can be described as a vessel which has provision of cell cultivation under sterile condition & control of environmental conditions e.g., pH, Temperature, Dissolved oxygen etc.  
  • It can be used for the cultivation of microbial plant or animal cells.
  • This process can either be aerobic or anaerobic. 
  • The bioreactors are commonly cylindrical, ranging in size from litres to cubic metres, and are often made of stainless steel.

Specifications of a bioreactor

A typical bioreactor consists of following parts:
  • Agitator – used for the mixing of the contents of the reactor which keeps the “cells” in the perfect homogenous condition for better transport of nutrients and oxygen to the desired product(s).
  • Baffle – used to break the vortex formation in the vessel, which is usually highly undesirable as it changes the center of gravity of the system and consumes additional power.
  • Sparger – In aerobic cultivation process, the purpose of the sparger is to supply adequate oxygen to the growing cells.
  • Jacket – The jacket provides the annular area for circulation of constant temperature of water which keeps the temperature of the bioreactor at a constant value
High productivity, high product yield and high product concentration are the major objectives of plant tissue process development. A variety of bioreactor types providing growth and expression of bioactive substances are available today for plant cell and tissue cultures. Low biomass and product level can be achieved in any type of bioreactors. However, an improved understanding of the manifold interactions between cultivated cells, product formation and the specific designs for different bioreactor types will enhance and sustain high productivity and also reduce the process costs.

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